Composite

Part:BBa_K2244011:Design

Designed by: Chen Hong   Group: iGEM17_SSTi-SZGD   (2017-10-25)

CloE promoter+TorA+opdA+T1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1286
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 578
    Illegal AgeI site found at 917
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This parts will work with 【CloE promoter+mcherry+Lev1+P+Lev1+T1 terminator【BBa K2244011】 in our system, the result will show the level of OPH enzyme production when the light-regulate gene expression system works.